FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n01_vTC35C2_P2CW_100d_rep1_03142019.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n01_vTC35C2_P2CW_100d_rep1_03142019.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2008540
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG62370.31052406225417467No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA41240.20532326963864297No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG36040.1794338175988529No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT35460.17654614794826093No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT31600.15732820854949367No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT28770.1432383721509156No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA28670.1427404980732273No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA27820.138508568412877No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC24690.12292510978123414No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT24640.12267617274239No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA24540.12217829866470171No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA23660.11779700678104493No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG23580.11739870751889432No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAA22980.11441146305276469No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT21180.10544972965437582No Hit
TTCTTACACTTTCCATGCATTCATTGTCACACTTGTGGTAGAACTCAAAA20810.10360759556692922No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA20370.10141694962510082No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA20260.10086928813964373No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT20200.10057056369303075No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA6600.0126.333631
GCAAAAG41250.0102.462853
GCGCGAA451.0917574E-896.672691
GCAGGGT25650.085.076929
GCAGGGG14300.081.624879
AGCAGGC5000.078.2990348
AGCAGGG44150.076.029648
AGCGAAA13250.072.230921
AGCAAAA61550.069.611542
CGCGAAA651.3991303E-766.9205862
AAGCAGG77400.065.848677
CAAAAGC69500.061.127224
GAGCAAA72750.058.9005781
ACAAGGG13600.058.1044543
AGGGTGT10600.056.7667546
CAAGGGT14200.055.6493384
TAAACTA3800.055.3268855
AAGGGTG11400.052.1471825
TATTAGT1301.3096724E-1050.195441
CTATTCG4200.050.0589039
Produced by FastQC (version 0.11.5)