FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n01_vTC35C1_P2CW_und_rep2_03142019.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n01_vTC35C1_P2CW_und_rep2_03142019.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1848311
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG53090.2872352109574633No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA40410.21863203757376326No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT39420.21327579611872677No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG31860.17237358864390245No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT25710.1390999674838271No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT24210.1309844501277112No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT23580.1275759328381425No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT23480.12703489834773476No Hit
CAATAGGAGAGTGCCCAAAATACGTCAGGAGTGCCAAATTGAGGATGGTT23310.12611513971404165No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA23220.12562820867267468No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC23090.12492486383514463No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA22890.12384279485432917No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA22170.11994734652339353No Hit
TTCTTACACTTTCCATGCATTCATTGTCACACTTGTGGTAGAACTCAAAA21140.11437469127219392No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA20580.11134489812591063No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA20060.10853151877579044No Hit
GAGTAGAGGCTTTGGGTCCGGCATCATCACCTCAAACGCATCAATGCATG19260.1042032428525286No Hit
CAATATGTATAGGCTACCATGCGAACAATTCAACCGACACTGTTGACACA19220.10398682905636551No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA18550.10036189797063373No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA6100.0112.925911
GCAAAAG39200.090.813053
GCAGGGG13400.081.698349
AGCGAAA12300.073.099841
GCAGGGT22800.072.8185049
AGCAGGG40450.068.107428
AGCAAAA53350.067.2721562
AGCAGGC4050.066.2332468
AGGGTGT8000.065.252236
AAGCAGG68850.061.7053767
TTAGTAG1350.059.0776862
CAAGGGT9950.059.022114
ACAAGGG10000.058.7270053
TCGTACT5700.058.5123562
AAGGGTG8950.058.326015
CAAAAGC62550.057.491974
GAGCAAA63250.056.6325721
TAAACTA2750.055.3655245
ATTAGTA1450.055.0078281
ACAGACT3600.052.3600658
Produced by FastQC (version 0.11.5)