FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n01_vTC35C1_P2CW_und_rep1_03142019.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n01_vTC35C1_P2CW_und_rep1_03142019.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2338559
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG57510.2459206716614804No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT47520.20320205733530775No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA46690.19965286315205216No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG39180.16753906999994442No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT31770.1358528906048554No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA30370.12986629800659297No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT30020.12836964985702734No Hit
CAATAGGAGAGTGCCCAAAATACGTCAGGAGTGCCAAATTGAGGATGGTT27340.11690960116892497No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT27110.11592608952778186No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA27080.11579780540067622No Hit
TTCTTACACTTTCCATGCATTCATTGTCACACTTGTGGTAGAACTCAAAA26870.11489981651093686No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT26750.11438668000251437No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA26250.11224861121742064No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC25610.10951188317250067No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA24020.10271282443590263No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA23600.1009168466564239No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA8300.0123.162821
GCAAAAG48800.095.8228763
GCAGGGT25350.079.507019
GCAGGGG19650.077.8499769
AGCAGGC4800.069.4777768
AGCGAAA16800.067.753071
AGCAGGG52500.067.665318
AGCAAAA69950.067.264562
AAGCAGG90100.063.486527
ACAAGGG12000.061.019613
TTAGTAG1700.059.704682
CAAAAGC80700.058.6636354
CAAGGGT12550.058.3454444
AGGGTGT10300.057.0134166
ATTAGTA1800.056.388961
GAGCAAA87250.055.0086631
TCGTACT6950.052.1572272
AAGGGTG11700.050.8109475
TAAACTA3600.046.3185165
ACTAATA1751.8189894E-1245.5705158
Produced by FastQC (version 0.11.5)