FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n01_vTC35C1_P2CW_100d_rep1_03142019.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n01_vTC35C1_P2CW_100d_rep1_03142019.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2028027
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG61690.30418727166847387No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA41220.20325173185564097No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG36170.17835068270787321No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT32100.15828191636501882No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT31970.1576408992582446No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA31200.1538441056258127No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT29770.14679291745129627No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC28830.14215787067923652No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA28030.13821315002216442No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT26020.1283020393712707No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA25420.1253434988784666No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA24960.12307528450065013No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG23690.11681304045754815No Hit
TTCTTACACTTTCCATGCATTCATTGTCACACTTGTGGTAGAACTCAAAA21920.10808534600377608No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT20880.10295720914958234No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT20800.1025627370838751No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA20400.10059037675533905No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA6900.0130.291671
GCAAAAG41850.0100.47933
AGCAGGC6300.081.7054758
GCAGGGT27350.078.993679
GCAGGGG13000.078.633779
AGCAGGG43650.073.080558
AGCAAAA58050.072.438572
AGCGAAA14450.070.2431951
CTACTAA1900.068.685176
AAGCAGG78050.065.950617
TAAACTA4200.063.869945
GGCGTAC350.003566835362.143721
GCGTACT350.003566835362.143722
CAAAAGC72500.058.4008144
CTATTCG4700.057.073869
AGGGTGT13250.056.9064526
GAGCAAA75350.055.999461
GCGCGAA651.0939571E-555.770011
ACAAGGG16550.054.759073
CGCGGGG400.006050265454.37844145
Produced by FastQC (version 0.11.5)