FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38555678
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29270517.591750818128526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG177000.014.1839135
TACACTG141150.013.5320295
CAAGGGT100700.012.860834
CTACACT119300.012.5538044
GTATTAG122050.012.3110541
GTCCTAG65750.012.0335411
GAGCAAA273250.011.900921
TAGACTG102150.011.544875
CTAGGAC64700.011.5186483
CTAGCCT101250.011.5047264
CTTACAC119550.011.4993313
CTAGTAC52200.011.3661113
GCAAAAG275500.011.1355793
TCTAGAC88900.011.0689323
CTATACT133300.010.9639414
GTGTAAG116800.010.9378641
TAGACAG182250.010.7979475
ACAGTGC115250.010.7970298
TAGTACT91850.010.7916254
CTAGACT90400.010.7246184
Produced by FastQC (version 0.11.5)