FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n01_RNA_MRTPCR_NC_03142019.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n01_RNA_MRTPCR_NC_03142019.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6877
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGAAAGCAGGACGAAGCTGCCGTTGCGTCCGCCAAGCGCACCCTGGAC821.1923803984295478No Hit
AAGCAGGACGAAGCTGCCGTTGCGTCCGCCAAGCGCACCCTGGACGGTTT410.5961901992147739No Hit
AGCGAAAGCAGGGGGATTCACAGGTGCGGGCGGTCAAAGACGGGAAGGCG370.5380253017304057No Hit
GCCCTGGACACTGCTTCGAAACGCGAGCATCTCGCCGTGGCTGACGGCTT350.5089428529882216No Hit
ATTCACAGGTGCGGGCGGTCAAAGACGGGAAGGCGATTATCCCTCAGGTC350.5089428529882216No Hit
GGCTTATAGTGCGGAGTGCCCATTCAACGAGGAGAGCGTTCATGAAACTG330.47986040424603754No Hit
GGACGAAGCTGCCGTTGCGTCCGCCAAGCGCACCCTGGACGGTTTCCATC330.47986040424603754No Hit
AAGCAGGGGGATTCACAGGTGCGGGCGGTCAAAGACGGGAAGGCGATTAT290.42169550676166934No Hit
CTATAAGCCAACGCGGTGCTGCCGGCGGTAACGGTTTGTCAGGTGCGCCG280.40715428239057727No Hit
GTCAAAGACGGGAAGGCGATTATCCCTCAGGTCGAATCCGGCATCCTCGA270.3926130580194852No Hit
GTCCAGGGCTCTGCGGGCTACGTGGCGATGGAAACCGTCCAGGGTGCGCT220.319906936164025No Hit
ATAGTGCGGAGTGCCCATTCAACGAGGAGAGCGTTCATGAAACTGCAGGC210.3053657117929329No Hit
CCCTGGACGGTTTCCATCGCCACGTAGCCCGCAGAGCCCTGGACACTGCT210.3053657117929329No Hit
GGTCGAATCCGGCATCCTCGATGCCGGCGCACCTGACAAACCGTTACCGC180.26174203867965684No Hit
GGTGCGGGCGGTCAAAGACGGGAAGGCGATTATCCCTCAGGTCGAATCCG180.26174203867965684No Hit
TCACAGGTGCGGGCGGTCAAAGACGGGAAGGCGATTATCCCTCAGGTCGA180.26174203867965684No Hit
TCCCTGAGCGCCGTCGCGGCAAGCAGCGGGCTTGGCCGCATGTCGCTCGA170.24720081430856478No Hit
AGCGAAAGCAGGGCAGGGGGAGAGGGGCTGGCCTCAAAGAGAGAGAGAGA160.23265958993747274No Hit
AAAGCAGGTTGACCACCGTCTGCGCAGTGCTGTTCAACCTGCTGGTCGAC160.23265958993747274No Hit
TTCCAGGTCGCCGTGAAATTGCTTGTCGAGCGACATGCGGCCAAGCCCGC160.23265958993747274No Hit
TCATGAAACTGCAGGCCAGCGGTCCGTTCGAAGTGAAGCTGAATCCGGAA150.21811836556638067No Hit
AGCGAAAGCAGGGGGTCGATGAATGGTGATATTTACACAGTAATGTAAGT150.21811836556638067No Hit
GTACGGGATCGCGTACATCATGTCGACGACGCGCATCATCACGGAGTCGA150.21811836556638067No Hit
AAGCAGGGCAGGGGGAGAGGGGCTGGCCTCAAAGAGAGAGAGAGAGAGAG150.21811836556638067No Hit
GTTGACCACCGTCTGCGCAGTGCTGTTCAACCTGCTGGTCGACCTCGCGT140.20357714119528864No Hit
AGCGAAAGCAGGTTGACCACCGTCTGCGCAGTGCTGTTCAACCTGCTGGT140.20357714119528864No Hit
GGGATTCACAGGTGCGGGCGGTCAAAGACGGGAAGGCGATTATCCCTCAG140.20357714119528864No Hit
GGCCAGCGGTCCGTTCGAAGTGAAGCTGAATCCGGAATCCCTGAGCGCCG140.20357714119528864No Hit
AAGCTGCCGTTGCGTCCGCCAAGCGCACCCTGGACGGTTTCCATCGCCAC130.1890359168241966No Hit
GCCGTTGCGTCCGCCAAGCGCACCCTGGACGGTTTCCATCGCCACGTAGC130.1890359168241966No Hit
CCTGACAAACCGTTACCGCCGGCAGCACCGCGTTGGCTTATAGTGCGGAG130.1890359168241966No Hit
ACATGCGGCCAAGCCCGCTGCTTGCCGCGACGGCGCTCAGGGATTCCGGA130.1890359168241966No Hit
CTGCCGTTGCGTCCGCCAAGCGCACCCTGGACGGTTTCCATCGCCACGTA130.1890359168241966No Hit
GTGTTGCAGGCCTGCTAGTTACCTCTGGCCCACTAGCTCAGAGCCCTGTG130.1890359168241966No Hit
ATACAAGGCTGACCACTGGGGTAATTACCAAAGCAGCCTCTCTTCCCCAC130.1890359168241966No Hit
AAAGCAGGGGGATTCACAGGTGCGGGCGGTCAAAGACGGGAAGGCGATTA130.1890359168241966No Hit
CCGTCTGCGCAGTGCTGTTCAACCTGCTGGTCGACCTCGCGTATGCGTGG130.1890359168241966No Hit
TCGCAAGCGCGAGCGGCGAGCGCGAACGCGGCGGCGAATCGGTCTGCACG120.17449469245310456No Hit
GACAAACCGTTACCGCCGGCAGCACCGCGTTGGCTTATAGTGCGGAGTGC120.17449469245310456No Hit
TTCATGAACGCTCTCCTCGTTGAATGGGCACTCCGCACTATAAGCCAACG120.17449469245310456No Hit
AGCGAAAGCAGGTACGGGATCGCGTACATCATGTCGACGACGCGCATCAT120.17449469245310456No Hit
GGCCAAGCCCGCTGCTTGCCGCGACGGCGCTCAGGGATTCCGGATTCAGC120.17449469245310456No Hit
AAGCAGGAGAGAGGACATCTAAATGCACAGTCCAATACAAGGCTGACCAC110.1599534680820125No Hit
AGTTACCTCTGGCCCACTAGCTCAGAGCCCTGTGGCTGTTTGTTGATCAT110.1599534680820125No Hit
GGCTGACGGCTTCCAGGTCGCCGTGAAATTGCTTGTCGAGCGACATGCGG110.1599534680820125No Hit
CCCTCAGGTCGAATCCGGCATCCTCGATGCCGGCGCACCTGACAAACCGT110.1599534680820125No Hit
ATTCCGGATTCAGCTTCACTTCGAACGGACCGCTGGCCTGCAGTTTCATG110.1599534680820125No Hit
GCGAAAGCAGGGGGATTCACAGGTGCGGGCGGTCAAAGACGGGAAGGCGA100.14541224371092046No Hit
TTATCCCTCAGGTCGAATCCGGCATCCTCGATGCCGGCGCACCTGACAAA100.14541224371092046No Hit
AGCGTGACCAGCGCCAGCAGCACGAGGCTGAACACGGCCGCGCGGTTGTG100.14541224371092046No Hit
ACCTGGAAGCCGTCAGCCACGGCGAGATGCTCGCGTTTCGAAGCAGTGTC100.14541224371092046No Hit
GGATTCACAGGTGCGGGCGGTCAAAGACGGGAAGGCGATTATCCCTCAGG100.14541224371092046No Hit
AAATATATGTTTGCACATCACTGTGTGTTGCAGGCCTGCTAGTTACCTCT100.14541224371092046No Hit
CTTCAGAGGTATGGCCCTTTGATGTGGGGAAGAGAGGCTGCTTTGGTAAT100.14541224371092046No Hit
AGCGAAAGCAGGGTCAAGGTTCAGAGGTCAAAGGTCACAGGTCAAGGGTC100.14541224371092046No Hit
CCGTTACCGCCGGCAGCACCGCGTTGGCTTATAGTGCGGAGTGCCCATTC100.14541224371092046No Hit
CTGCAGGCCAGCGGTCCGTTCGAAGTGAAGCTGAATCCGGAATCCCTGAG90.13087101933982842No Hit
CTCGATGCCGGCGCACCTGACAAACCGTTACCGCCGGCAGCACCGCGTTG90.13087101933982842No Hit
GCCACGTAGCCCGCAGAGCCCTGGACACTGCTTCGAAACGCGAGCATCTC90.13087101933982842No Hit
GCACATCACTGTGTGTTGCAGGCCTGCTAGTTACCTCTGGCCCACTAGCT90.13087101933982842No Hit
CTTCACAACCGCGCGGCCGTGTTCAGCCTCGTGCTGCTGGCGCTGGTCAC90.13087101933982842No Hit
TGCGTGGCTCGATCCGCGCATCCGTTATTGAGCGAGGCACCGCGATGACT90.13087101933982842No Hit
ACTTCGAACGGACCGCTGGCCTGCAGTTTCATGAACGCTCTCCTCGTTGA90.13087101933982842No Hit
CCTCGATGCCGGCGCACCTGACAAACCGTTACCGCCGGCAGCACCGCGTT90.13087101933982842No Hit
GTTTCCATCGCCACGTAGCCCGCAGAGCCCTGGACACTGCTTCGAAACGC90.13087101933982842No Hit
GTGTACACTGACAGAAGCACAAATGGGACTTACACTACCAGGACGTGCAA90.13087101933982842No Hit
AACGAGGAGAGCGTTCATGAAACTGCAGGCCAGCGGTCCGTTCGAAGTGA90.13087101933982842No Hit
GGTACGGGATCGCGTACATCATGTCGACGACGCGCATCATCACGGAGTCG90.13087101933982842No Hit
GGTCACGCTCGCGTGCTTCGTCGGCCCGTGGCTGCTCGCGGCCGATCCCG90.13087101933982842No Hit
GAGCGAAAGCAGGGGGATTCACAGGTGCGGGCGGTCAAAGACGGGAAGGC80.11632979496873637No Hit
TCGCTCGACAAGCAATTTCACGGCGACCTGGAAGCCGTCAGCCACGGCGA80.11632979496873637No Hit
AGCGAAAGCAGGCAGAGAGCTAATAGCTGAAAACCCTTTTTTCTCCAGTA80.11632979496873637No Hit
CGCCAAGCGCACCCTGGACGGTTTCCATCGCCACGTAGCCCGCAGAGCCC80.11632979496873637No Hit
GTGTAGTGGACTGTAGGAGATTTAGAGGGGTCAGAGTCGGGGTCAGAGTC80.11632979496873637No Hit
CGCGAGCATCTCGCCGTGGCTGACGGCTTCCAGGTCGCCGTGAAATTGCT80.11632979496873637No Hit
CCAGCGGTCCGTTCGAAGTGAAGCTGAATCCGGAATCCCTGAGCGCCGTC80.11632979496873637No Hit
GCTTCACTTCGAACGGACCGCTGGCCTGCAGTTTCATGAACGCTCTCCTC80.11632979496873637No Hit
GGGCAGGGGGAGAGGGGCTGGCCTCAAAGAGAGAGAGAGAGAGAGAGAGA80.11632979496873637No Hit
TCACTGTGTGTTGCAGGCCTGCTAGTTACCTCTGGCCCACTAGCTCAGAG80.11632979496873637No Hit
GCGAAAGCAGGACGAAGCTGCCGTTGCGTCCGCCAAGCGCACCCTGGACG80.11632979496873637No Hit
TTTCGAAGCAGTGTCCAGGGCTCTGCGGGCTACGTGGCGATGGAAACCGT80.11632979496873637No Hit
CTGCTTCGAAACGCGAGCATCTCGCCGTGGCTGACGGCTTCCAGGTCGCC80.11632979496873637No Hit
TCATCACGGAGTCGACGCGGCCGCCGGCGAAGCCCGCAGTCGCGCCCCAC80.11632979496873637No Hit
CCTCGTTGAATGGGCACTCCGCACTATAAGCCAACGCGGTGCTGCCGGCG80.11632979496873637No Hit
CGCCGTGGCTGACGGCTTCCAGGTCGCCGTGAAATTGCTTGTCGAGCGAC80.11632979496873637No Hit
GCGCTCAGGGATTCCGGATTCAGCTTCACTTCGAACGGACCGCTGGCCTG80.11632979496873637No Hit
GCTTCGAAACGCGAGCATCTCGCCGTGGCTGACGGCTTCCAGGTCGCCGT80.11632979496873637No Hit
TATCAATATGCATTCTGAAAAGCCCTGAATCTGTTTAGGTGTCTGACTGG80.11632979496873637No Hit
TTACACAGTAATGTAAGTAACTCCAGTCAGACACCTAAACAGATTCAGGG80.11632979496873637No Hit
ACCTGATACTTCATCAACCGAGACACTGTTGTGACAGGAAGTGTGTGTGT80.11632979496873637No Hit
GGTTGACCACCGTCTGCGCAGTGCTGTTCAACCTGCTGGTCGACCTCGCG70.10178857059764432No Hit
GTCCAATACAAGGCTGACCACTGGGGTAATTACCAAAGCAGCCTCTCTTC70.10178857059764432No Hit
ATTTCACGGCGACCTGGAAGCCGTCAGCCACGGCGAGATGCTCGCGTTTC70.10178857059764432No Hit
GTAATGTAAGTAACTCCAGTCAGACACCTAAACAGATTCAGGGCTTTTCA70.10178857059764432No Hit
GCCCGCTGCTTGCCGCGACGGCGCTCAGGGATTCCGGATTCAGCTTCACT70.10178857059764432No Hit
ACGCCGAACAGGCCGGACACGAGCGTGCCGAGCACGCCGACCTCGATCGA70.10178857059764432No Hit
GTCGAGCGACATGCGGCCAAGCCCGCTGCTTGCCGCGACGGCGCTCAGGG70.10178857059764432No Hit
CCACGTAGCCCGCAGAGCCCTGGACACTGCTTCGAAACGCGAGCATCTCG70.10178857059764432No Hit
TCGCCACGTAGCCCGCAGAGCCCTGGACACTGCTTCGAAACGCGAGCATC70.10178857059764432No Hit
CAGGGATTCCGGATTCAGCTTCACTTCGAACGGACCGCTGGCCTGCAGTT70.10178857059764432No Hit
GTTTAGGTGTCTGACTGGAGTTACTTACATTACTGTGTAAATATCACCAT70.10178857059764432No Hit
CCTCTGGCCCACTAGCTCAGAGCCCTGTGGCTGTTTGTTGATCATGTTCT70.10178857059764432No Hit
AATCCGGCATCCTCGATGCCGGCGCACCTGACAAACCGTTACCGCCGGCA70.10178857059764432No Hit
GGTCAAGGGTCAAACCTTTTGGACCAAGGTCATTTCACACAGCACACACA70.10178857059764432No Hit
CATTCAACGAGGAGAGCGTTCATGAAACTGCAGGCCAGCGGTCCGTTCGA70.10178857059764432No Hit
GGCCTGCAGTTTCATGAACGCTCTCCTCGTTGAATGGGCACTCCGCACTA70.10178857059764432No Hit
GCGTACATCATGTCGACGACGCGCATCATCACGGAGTCGACGCGGCCGCC70.10178857059764432No Hit
GAGCGAAAGCAGGACGAAGCTGCCGTTGCGTCCGCCAAGCGCACCCTGGA70.10178857059764432No Hit
GTGTATGTATGTGTGTTTGTGCGCGCGTATGTGTTTGTGTGGGTATGGGT70.10178857059764432No Hit
TTCAACGAGGAGAGCGTTCATGAAACTGCAGGCCAGCGGTCCGTTCGAAG70.10178857059764432No Hit
GGTCAAAGGTCACAGGTCAAGGGTCAAACCTTTTGGACCAAGGTCATTTC70.10178857059764432No Hit
GCAGGACGAAGCTGCCGTTGCGTCCGCCAAGCGCACCCTGGACGGTTTCC70.10178857059764432No Hit
CACACATACGTCCACAAAAAACAAAAAATGGTGTTCACACGTATCAATAT70.10178857059764432No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA151.17777134E-4145.01
GCGAAAG203.702467E-4108.752
CGAAAGC203.702467E-4108.753
GAAAGCA258.990981E-487.04
AAGCAGG659.827487E-655.769232
AGCAGGG400.005798514554.3753
AAAGCAG701.5243975E-551.7857131
GGGGGGG25150.06.860835140-144
AAAAAAA6102.5465852E-116.6557374140-144
Produced by FastQC (version 0.11.5)