FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n01_Plasmid_control_NA_03142019.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n01_Plasmid_control_NA_03142019.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1543275
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCATGTACTGTGTCATTTGAATGCACGTTGTTTAGTGTTGTTCCCTGTC85120.55155432440751No Hit
GTACATGATAGGACCCCTTATCGGACCCTATTGATGAATGAGTTAGGTGT71820.46537396121883656No Hit
TTCATATACTGATGACCTCTTTATAGCCAACCTTTGTTCATGGCAGCCAG53980.34977563946801443No Hit
GTATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCT50260.32567105668140806No Hit
ATTAATAACTAGTCAATAATCAATGTCGACCCAGGTGGCACTTTTCGGGG41410.2683254766648847No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG35660.23106704897053343No Hit
GTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATG33860.21940354117056263No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC31630.20495375095170984No Hit
TGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCC29670.19225348690285268No Hit
TACTAGCACATTGTCAGGAAGTGCTCAGCATGTCGAGGAGTGCTCCTGCT29480.19102233885730022No Hit
ATCTACAAGCCTCCCATTGTAAATGAAGCTAGCAGTTGCATTTTTATCAT28470.18447781503620547No Hit
GTTTAGTGTTGTTCCCTGTCCAAGGGCAAATTGATAACACTTGTCAGGAT28110.18214511347621132No Hit
AGCTTATATAGGCATGAGATTGATGTCCGCCCCATCAGGCCATGAGCCTG28060.18182112714843435No Hit
ATATAAGCTTTCGCAATTTTAGAAAAAAACTCCTTGTTTCTACTAATAAC28010.18149714082065738No Hit
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA27930.1809787626962142No Hit
TTCATATCCTGAGCGTAACTTCTCGCTGATCGTTCTTCCCATCCACACGT26770.17346227989178858No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA26730.17320309082956697No Hit
ATCCTGACAAGTGTTATCAATTTGCCCTTGGACAGGGAACAACACTAAAC24280.15732776076849556No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT24140.15642059905072006No Hit
GTCATGTACTGGGCATAATGCCAGGCGGGCCATTTACCGTCATTGACGTC24110.15622620725405387No Hit
GTAGTAATGACTGATGGGAGTGCTGATACTAAAATACTATTCATTGAGGA23740.1538287084285043No Hit
TCATTGAGGAGGGGAAAATCGTTCATACTAGCACATTGTCAGGAAGTGCT22470.14559945570296934No Hit
ATATGAAACAGCCCCCTGCTGTCCATTCCTTATTCCATAGAAAAGCCTTG22360.14488668578186No Hit
CTTGATAACTACTGTAACATTGCATTTCAAGCAATATGAATTCAACTCCC22000.14255398422186583No Hit
GACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCCCATAAGGTCATGT22000.14255398422186583No Hit
CTACTGTAACATTGCATTTCAAGCAATATGAATTCAACTCCCCCCCAAAC21980.14242438969075505No Hit
CCCATATGTCCTTCCGAGTGAGAGACACAAAAAATTCCAACACACTATTG21890.14184121430075652No Hit
GTATTGTTTCATGGTCTAAAGAAATCCTCAGGACCCAGGAGTCAGAATGC21750.140934052582981No Hit
GTTCCATATGTACCTGAGGTGCCACAAAACACAACAATACTGTTTGAGGT21220.13749979750854513No Hit
GTCTAAAGAAATCCTCAGGACCCAGGAGTCAGAATGCGTTTGTATCAATG21010.1361390549318819No Hit
CTAAAATACTATTCATTGAGGAGGGGAAAATCGTTCATACTAGCACATTG20480.13270479985744602No Hit
TTGCAATAGTGTGTTGGAATTTTTTGTGTCTCTCACTCGGAAGGACATAT19780.12816899126856848No Hit
ATCTTATCATGTCTGGATCCGCTGCATTAATGAATCAGGGGATAACGCAG19770.12810419400301307No Hit
GTCAGGAAGTGCTCAGCATGTCGAGGAGTGCTCCTGCTATCCTCGATATC19530.12654905962968363No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT19500.12635466783301746No Hit
CTTTATAGCCAACCTTTGTTCATGGCAGCCAGCATATGGGCATATGTTGC19320.12518831705302036No Hit
TCTTTAGACCATGAAACAATACTATCTACAAGCCTCCCATTGTAAATGAA19100.12376277721080171No Hit
CTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGT19090.1236979799452463No Hit
CCATAAGAGAAGAGGGACAGCTATGACTGGGAGTAGTCAGGAGAGGAGGA18830.12201325104080607No Hit
ATGATACACTTGATGTACTGCCAAGTGGGCAGTTTACCGTAAATACTCCA18710.12123568385414135No Hit
CCTCAATGAATAGTATTTTAGTATCAGCACTCCCATCAGTCATTACTACT18530.12006933307414427No Hit
GTATTTTAGTATCAGCACTCCCATCAGTCATTACTACTGTACAAGTTCCA18230.11812541510748246No Hit
TTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGT18110.11734784792081775No Hit
CTCCATAAGAGAAGAGGGACAGCTATGACTGGGAGTAGTCAGGAGAGGAG17820.11546872721971133No Hit
TATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTG17560.1137839983152711No Hit
ATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAA17480.11326562019082795No Hit
AGTAATGACTGATGGGAGTGCTGATACTAAAATACTATTCATTGAGGAGG17280.11196967487972008No Hit
ACACTAAACAACGTGCATTCAAATGACACAGTACATGATAGGACCCCTTA17040.11041454050639062No Hit
CATTACTACTGTACAAGTTCCATTGATACAAACGCATTCTGACTCCTGGG16980.11002575691305826No Hit
GTGCTAGTATGAACGATTTTCCCCTCCTCAATGAATAGTATTTTAGTATC16860.10924818972639354No Hit
ATACTATCTACAAGCCTCCCATTGTAAATGAAGCTAGCAGTTGCATTTTT16850.10918339246083816No Hit
GGTAATAGGTCCGGTTATTCTGGTATTTTCTCTGTTGAAGGCAAAAGCTG16400.10626751551084544No Hit
CTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTG16290.10555474558973611No Hit
CTTCCTGACAATGTGCTAGTATGAACGATTTTCCCCTCCTCAATGAATAG16260.10536035379306993No Hit
CAATAATAGAAAGAAACATAACAGAGATAGTGTATCTGACCAACACCACC16090.10425880027862824No Hit
GTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT16040.10393481395085126No Hit
ATTGGAGCCTTTCCAGTTGTCTCTGCAGACACATCTGACACCAGGATATC16030.10387001668529589No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGCG608.0322934E-872.50293145
ATACTGA18800.057.080476
CTGATGA18400.056.737759
TACTGAT19050.055.943527
TATACTG19550.055.2615665
CATATAC20550.052.2196043
ACTGTGT30850.044.4154478
TACTGTG31200.043.6848347
CTGTGTC31650.043.5218479
TTTCGTC1002.7343522E-643.50176145
ATGATAG30400.041.024055
TCTCGTT907.482552E-540.279404145
GATAGGA30550.040.105417
GGAGACC1451.9283107E-840.001617145
TGATAGG31500.039.591476
ATAGGAC31150.039.3329128
TAGGACC31050.039.2260979
ATATACT40450.039.077044
GTACTGT36000.037.8650936
ACTAGTC24350.034.5371448
Produced by FastQC (version 0.11.5)