FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n01_No_index_control_03142019.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n01_No_index_control_03142019.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66
Sequences flagged as poor quality0
Sequence length151
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTACGCGGGAGACGTGTGCTCTTCCGATCTTCTATGGGAGACGTGTGCT11.5151515151515151Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GACCCGGAGACGTGTGCTCTTCCGATCTCCCCGGGGCTGTCTCTTATACA11.5151515151515151Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTATCATCGCTACGTCTTCTTCCTGAGATGGCTAACCCTTTCGCTCCT11.5151515151515151No Hit
GTGCTCTTCCGATCTGAGAGGGGGTGTGCTCTTCCGAAAAAAAAAAAAAA11.5151515151515151No Hit
CTACTAAGATCGGAAGAGCTCACGTCTCCCTCTGTCTCTTATACACATCT11.5151515151515151Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CGCGGGAAGGGTCGCCGTGGGAGAAGGGGGACTCCCCGGAAACCCGGCGG11.5151515151515151No Hit
TACAAGAACATGGGCCTTGAAACCTGGCCTATGGACCTTCTTATAAGCTG11.5151515151515151No Hit
GCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTATGGTACGGCCCCCC11.5151515151515151No Hit
GGGCAGCAATGGCGCGGGCAGCTTGCGGCGCTCGAAGTCCTCGGGGCTCA11.5151515151515151No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAGGC11.5151515151515151No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTAGCCCCCACGCCCCC11.5151515151515151No Hit
GCGGGATTTGGCGCGGGATGCGGGGGTTCGGGTTCCGGGGCCTTTACTAT11.5151515151515151No Hit
GATGAAATTGGACTTCTAACCTCGGACCATGATCTGGTTTAGGGACCTGT11.5151515151515151No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTAGTTGAGC11.5151515151515151No Hit
GGAGACGTGTACTCTTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.5151515151515151No Hit
GTGCTCGTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGCACC11.5151515151515151No Hit
GCTCAACCGTGCGGGCACGCATACTGCGCAGGTACCCAAGCTGAACGAAT11.5151515151515151No Hit
GGCTGTAGACTGCCCCAGATGTTCAGTCAGCGGAGAAAGAGTCTACTAAC11.5151515151515151No Hit
CTGCGGACGCGGCCTGCTGTCAGACCATGATGCTGCGGATAGAGTGGCGA11.5151515151515151No Hit
GCACCAAGCACCCAGGCGACGACGGCAACGAAGCGGCGAGCCGGGCGATT11.5151515151515151No Hit
TCGTCGGCCACGCCATCGGCGGCATCGTCGGCGTCGAGCTTGCGCTGCGT11.5151515151515151No Hit
GGGTGGATGGCATGCCATTCTGCCGCATTTGAAGATCTAAGAGTATTAAG11.5151515151515151No Hit
AGAACACACACCCCTGTGAGATCGGAAGAGCACCTGGCTCTTATACACAT11.5151515151515151No Hit
AGGTAGAGCACTGGATGGTCTAGGGGGCTTATCGGCTTACCGAAATCAGC11.5151515151515151No Hit
GTGGAAGAAGATCGTGATCGGCATGGACGTCAGTTATGCTCCCTGGGCAT11.5151515151515151No Hit
ACTTTGAGTTTGAAAGGGTGCGCCTCGAGGTTCTCCTGGAAATCGGCGTG11.5151515151515151No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTAGGAAGTGACC11.5151515151515151No Hit
CGATGGCGGCCTGCTCAATCTCGGCGGCGACACCGCAGGCAACGGACCGC11.5151515151515151No Hit
GATTGGAAGAGCACACGTCTCCCTAAAGAGATCGGAAGAGCACACGTCTC11.5151515151515151Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATAGC11.5151515151515151No Hit
CATTGGCAAAGGGTGCTTGAAAGAAAGTTTTAAATTAGGTGTTTTCTTTC11.5151515151515151No Hit
ACTCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGTCTT11.5151515151515151No Hit
CTCTAGAGGGAGGCGTGTGCTCTTCCGATCTGGCGAGGGAGACGTATGCC11.5151515151515151Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAGCACACGTCTCCCAGTGGAGATCGGGAGAGCACACGTCTCCCAGCTGT11.5151515151515151Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTGTAATGGGAGACGTGTGCTCTTCCGATCTGAATTGGGAGACGTGTGCT11.5151515151515151Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CTTATAAACTCATAGTAGCTCTCAGAAGTTACCACAAAGCCGTATGGCGC11.5151515151515151No Hit
CCGCTGGGCTGCGCAGCAGCCCCAAAATCCTGGGAGCGCTTCGCACTCCA11.5151515151515151No Hit
ACCACGCATCGGCGGCGCGCCACCGTCTTGGCCATCGGGTTGGACACGCC11.5151515151515151No Hit
GCTCTTCCGATCTGATTGGGGTGGCCACCCCACCAGGGCAAAAGCCGGGA11.5151515151515151No Hit
ACCTCCCCCCAGGTCCCTCCTATGACATGTGGGTATTATAGGAGCTACAG11.5151515151515151No Hit
CTCACACACAGCATGTTAGTCTCCCTTACACCCCAGAACCCCTAATGTCT11.5151515151515151No Hit
AAGCACACGTCTCCCAGCAGAGATCGGAAGAGCACACGTCTCCCGGTCTA11.5151515151515151Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AATCAGATCGGAAGAGCACACGTCTCCCTTTCTAGCTGTCTCTTATACAC11.5151515151515151Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGTGGGGGCCGTCACCATCACAGCTCAAGCGGAAGAGGCTGGACGACCAT11.5151515151515151No Hit
ATCCTGTACTGTCGAGAAAAGCTTCGTGAGCGAGGTGGCGGGTCCGCCCG11.5151515151515151No Hit
TGTATGCGTAGCGGTGTACTTAAGCCCCAGTAAACGGCGGTGGTAACTAT11.5151515151515151No Hit
CTCTGTGATCCTTAGTTTTTCTTTCTTCCTCTGAAAAGTTGAATAGCTGT11.5151515151515151No Hit
TCCTTGATGACCTCGCCCTTGTAGCCGCAGAACACGACGAAGTCCTTGAT11.5151515151515151No Hit
GTGCTCTTCCGATCTGGGACGGGGCGGCCGACGCCGCCGGCGGCACTGGC11.5151515151515151No Hit
AGGATGTATTTTACTTAAAAATAGCAAAGCTGGCTCTGGCATATCTCTAT11.5151515151515151No Hit
CTCTTCCGATCTGGTCTGGGAGACGTGTGCTCTTCCGATCTGGTCTGGGA11.5151515151515151Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GGAGACAGAGGGACCGGCTGGAAGAAGCCACGGGTCTGACTCCCTTCTCC11.5151515151515151No Hit
CGTCATGGAGGTCCAGCCGACCATGACCGAGCGCGACGCGCCGGTGGTCA11.5151515151515151No Hit
GCGCACATGAAGGCAAGCTGGATATTCTCGGCCCACGACGTGGTGATCAA11.5151515151515151No Hit
GTGCTCTTCCGATCTGGACAGGGTGCTCTTCCTGTCTCTTATACACATCT11.5151515151515151No Hit
ATGTATAGGCTACCATGCGAACAATTCAACCGACACTGATGACCTGTCTC11.5151515151515151No Hit
GTGCTCTTCCGATCTAAACCGGGGGTGTGCTCTTCCGATCCTGTCTCTTA11.5151515151515151No Hit
CTCCAAGTTCGTTTGGAATTTCTCCGCTACCCACAAGTCATCCAAGCACT11.5151515151515151No Hit
TCCGATCTGATGAGGGGTGTGCTCTTCCTAAAAAAAAAAAAAAAAAAAAA11.5151515151515151No Hit
ATGAAGGGTTGGTCTCCAGAGTATATAAAGAATACCTAAAACTCAACATA11.5151515151515151No Hit
GATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGCCCCCCGGG11.5151515151515151No Hit
GGATAGGGAGACGTGTGCTCTTCAGACGTGTGCTCTTCCGATCTAAGTTG11.5151515151515151Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
ACGGCAATGTGCGGACCGCATGTGTGACCTATGACACAGTCGGCGATCCC11.5151515151515151No Hit
GAGCACACGTCTCCCTTTTGAGATCGGAAGAGCACACGTCTCACTTTTGA11.5151515151515151Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
AAGTGAGAGAGAGCCGGAACCCAGGGAATGCTGAGTTCGAAGATCTCACT11.5151515151515151No Hit
TCCCTGCTCAGATCGGAAGAGCACACGTCTCCCTGCTCAGATCCTGTCTC11.5151515151515151Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCATG50.0145.01
GAGGTCC50.0145.08
GTCATGG50.0145.02
CATCTCG50.0145.0145
TGGAGGT50.0145.06
ATGGAGG50.0145.05
TCATGGA50.0145.03
AGGTCCA50.0145.09
GGAGGTC50.0145.07
CATGGAG50.0145.04