FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n01_MG_9424.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n01_MG_9424.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14559174
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG37400.018.0282235
TATACTG161150.016.6911095
TACACTG116700.015.9662835
TATACCG24900.015.1406865
CTACACT87200.014.7994334
CCTACAC71200.014.6629913
CTATACT105950.014.643824
GTGTAGG70500.014.2950811
CCTATAC61550.013.7815453
GTATAAG97850.013.7079291
CTTACAC92000.013.3968023
GTATAGA109400.013.38736251
TCTATAC102500.013.2976253
GTATTAG102850.013.1825151
TATACAG135750.013.1382295
TACACAG144250.013.1179585
GTATAGG63750.013.0791231
GTACTAG47450.012.8352411
GTCCTAG53750.012.8146311
TAGACTG86550.012.7326295
Produced by FastQC (version 0.11.5)