FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n01_MG_8724_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n01_MG_8724_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13934668
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG154100.015.8079965
GTATAGG59050.015.2250081
TACACTG119350.014.9435195
CTACACT88200.014.5493924
CCTACAC65050.014.2659683
TATACCG23000.014.1848535
CTATACT111700.013.6303274
GTGTTAG100700.013.4638071
TCTATAC105850.013.4246763
TACACCG25550.013.3366615
GTGTAGG65500.013.0616021
GTATTAG105900.012.7342311
GTATAGA102350.012.6800491
TAGACTG91950.012.5367675
ACTGATC87500.012.4284548
GCTATAC53850.012.3862753
TACACAG139150.012.3482475
ATACTGT199500.012.3196116
TAGGACT77000.012.1461644
GTCCTAG56850.012.115711
Produced by FastQC (version 0.11.5)