FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n01_MG_8724.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n01_MG_8724.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13665259
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG88000.016.065275
TACACCG38500.015.8181115
CCTACAC64100.015.7214023
CTATACT89150.015.4514184
CTACACT73450.015.0033374
TATACTG118200.014.5980745
TCTATAC93500.013.8020273
GTACTAG40500.013.7847971
TAGACAG120000.013.593695
CTACCCT58800.013.5628644
GTATAGG70950.013.1826271
TAGACTG74400.013.1551825
TACACAG108300.013.0539585
GTATAAG85700.013.0288051
CTAGACT58350.012.9219644
GTCCTAG46250.012.8548431
CTACACA95850.012.7829384
TATACAG114800.012.6937585
GTGTAGG64600.012.6826681
GTGTTAG81400.012.5591261
Produced by FastQC (version 0.11.5)