FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n01_MG_8721.TR2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n01_MG_8721.TR2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11720783
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG101000.016.725235
TATACTG131850.016.0561085
TACACCG20900.015.2631435
GTATTAG84800.014.9621711
GTGTAGG53400.014.7991791
TATACCG15900.014.591185
CTACACT75350.014.2401984
CTATACT86000.014.0784754
GTATAAG80200.013.8315061
TCTATAC84900.013.6631183
GTATAGG48750.013.5337811
GTATAGA87800.013.4600121
TAGACTG78300.013.4259125
CCTATAC47800.013.1955943
GTGTAGA74900.013.1646551
GTAGGAC36350.013.1636723
GTCCTAG52900.013.1573351
CTAGGAC48700.013.1006033
TACACAG122000.013.0737565
ATACTGT163000.012.9432946
Produced by FastQC (version 0.11.5)