FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n01_MG_8404.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n01_MG_8404.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13705941
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG140750.016.5862715
CTATACT97750.015.5013944
GTATAGA97250.015.2089511
CTAGGAC57300.015.183363
GTCCTAG58000.015.125721
TACACTG109700.014.8702125
TAGCGCG42050.014.484135145
CTACACT85550.014.4068884
CCTATAC58800.014.0562283
GTGTAGG64450.013.7244711
TCTATAC96250.013.7091933
CTAACAC94600.013.4118353
CCTACAC67200.013.37807753
GTACTAG44150.013.3018791
GTATAGG55650.013.0284731
TAGGACT79050.012.9317864
GTGTAGA85750.012.6828191
GTATTAG99700.012.5808391
GTGTAGC64200.012.5356441
GTGTTAG89250.012.51041
Produced by FastQC (version 0.11.5)