FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n01_MG_7379.TR3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n01_MG_7379.TR3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19169364
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG112300.017.1730185
TACACCG89750.015.5099895
TATACTG182000.015.3766115
CTACACT109950.014.5727584
GTATAGG112750.014.4686771
GTGTAGG100100.014.1966151
CCTATAC111750.014.1433593
CCTACAC105300.014.1146163
TAGACAG140800.014.0058985
CTATACT165800.013.9055224
GTATAAG156350.013.5409091
GTATAGA173450.013.5017811
TCTATAC173400.013.3796413
TATACCG74300.013.3683095
TAGACTG86150.013.2967825
ACACCGT81600.013.14971356
TCTACAC132400.013.0326443
GTGTATG119900.012.6383371
TACACAG133500.012.5994455
CTACACC154400.012.4904784
Produced by FastQC (version 0.11.5)