FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n01_MG_6331.TR3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n01_MG_6331.TR3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16491857
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG187600.015.5356495
TACACTG138250.015.2603355
AGACCGC53900.014.931314145
GTATAGA125900.014.454441
CTACACT107950.014.0365324
TATACAG165500.013.8428425
GTATTAG120050.013.8301421
GTGTAGG74200.013.386591
CTAGGAC70600.013.3497633
TACACAG177700.013.2596515
CCTATAC72700.013.0638683
CTCCGAC31050.012.842954145
GTGTAGA105950.012.7965811
ATACTGT240050.012.5942356
GTATAGG70000.012.5325831
TAGACTG106850.012.4847395
CTTACAC108100.012.4074033
GCTACAC68550.012.374093
GTGTAAG107200.012.3092031
TAGACAG213500.012.2926885
Produced by FastQC (version 0.11.5)