FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n01_MG_6119.TR2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n01_MG_6119.TR2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12699065
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG151150.016.0207775
GTACTAG41000.014.6776521
TACACTG110650.014.6116315
ATGCCGG44850.014.22661145
CTATACT95350.014.142874
CGTACAC15050.013.9702243
GTGTAAG87600.013.5738211
GTAGGAC37000.013.520393
ATACTGT190700.013.4205716
GTATAGC52400.013.0064551
TACACAG138000.012.87159255
CTAGGAC53250.012.6620853
TCTATAC99900.012.5551663
TAGACAG169300.012.4618155
GTATAAG88500.012.4526761
TAGGACC38650.012.1929594
GTGTAGG56050.012.159471
GTATTAG92900.012.0970191
GTATAGG50550.012.0481541
CTAACAC96650.012.0021763
Produced by FastQC (version 0.11.5)