FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n01_MG_5764.TR3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n01_MG_5764.TR3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20281227
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG256350.016.1490965
TACACTG176900.015.9019115
CTATACT162150.014.8892464
CTACACT134150.014.429994
GTATAGA161750.013.7165551
AGACGGC111750.013.56025145
ATACTGT326000.013.4994646
TCTATAC150850.013.4092113
GTATAGG88400.013.12307551
GTGTAGG94500.013.119951
TACACAG227850.013.1097255
TACACCG27900.012.99305255
GTGTAGA137750.012.7903551
CCTACAC100550.012.7624943
CTTACAC134950.012.57150653
CCTATAC85750.012.4287533
TATACAG210650.012.252765
TGCACTG201200.012.2156665
GTACTAG67050.012.1111991
TAGACTG138450.012.0966315
Produced by FastQC (version 0.11.5)