FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n01_MG_5764.TR2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n01_MG_5764.TR2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20572212
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACT119200.015.8136884
TATACTG208000.015.3016175
TACACTG136800.015.2630925
CCTACAC106000.014.5683333
CTATACT153050.014.5425694
TACACCG66750.014.445635
GTGTAGG102250.014.1819351
CTAGTAC56450.013.9990533
AGACGCG67450.013.974306145
TATACAG198200.013.6074085
GTATAGA166450.013.2421531
GTATTAG157650.013.1074951
GTGTAGA137250.013.04828551
GTGTAAG122600.012.9515721
CTAGGAC61750.012.9149243
TACACAG169950.012.8404865
TAGACAG189100.012.6136415
CTTACAC118600.012.5926933
CTACACC135700.012.5017874
TCTATAC159200.012.3868813
Produced by FastQC (version 0.11.5)