FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n01_MG_4340.TR3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n01_MG_4340.TR3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17965805
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG127250.015.897165145
CTCCGAC36800.015.17107145
TACACTG147450.014.8982795
TATACTG200500.014.5000085
GTATTAG127850.014.4042471
CTATACT125250.014.2974144
GTATAGA126150.013.8511991
TCTATAC123750.013.7090223
GTGTAGA105050.013.6655371
GTGTAAG114350.013.50521
CTTACAC108500.013.4308083
CTAGGAC75600.013.4258593
TACACAG177100.013.3046385
CCTATAC72700.013.163623
CTAGTAC54500.013.0366323
GTGTTAG126350.013.0259131
GTCCTAG76750.012.9419521
TAGACAG218850.012.8866875
GTGTAGG77150.012.7808741
GTATAGG66950.012.7787691