FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n01_MG_4329.TR2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n01_MG_4329.TR2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13658372
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG142850.017.2558695
TACACCG40400.015.2537195
TACACTG106150.015.0942125
CTATACT98000.014.8699044
TAGACCG21250.014.6705945
CTAGGAC45350.014.0683623
CCTACAC68350.014.0014693
TATACCG28600.013.9423145
GTGTAAG86700.013.7148651
GTCCTAG46600.013.6918951
TCTATAC105000.013.6714343
TAGACAG136950.013.4994575
GTGTAGG70250.013.417261
GTATTAG105250.013.3643061
GTATAGG61800.013.2573511
GTAGGAC45950.013.2535423
TCTACAC97400.013.1006213
TATACAG134100.013.0835245
GTATAGA109250.013.0077281
CTACACT82900.012.8558554
Produced by FastQC (version 0.11.5)