FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n01_MG_341.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n01_MG_341.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19449838
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG174550.016.9051695
TATACTG230850.016.2998075
CTACACT124100.015.4817154
CCTACAC90500.015.2211983
CCTATAC78250.014.546523
GTATTAG142100.013.6228071
TAGACTG134750.013.5586585
CTAGGAC86850.013.5234983
ATGCCGG52050.013.512063145
TACACAG217050.013.3945935
CTATACT147800.013.3916214
CTCCGAC29100.013.20546145
GTGTAGG88750.013.1524581
GTGTAAG131300.013.1420061
CTTACAC132500.013.1322513
GTATAGG79050.013.0237451
GTAGGAC60350.012.9744913
CTAGACT116850.012.9676884
TAGGACT117600.012.8233354
TCTACAC122150.012.7611223
Produced by FastQC (version 0.11.5)