FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n01_MG_30908.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n01_MG_30908.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13239886
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG14000.016.5715245
GTATTAG86750.015.5453451
TATACTG160500.015.2228295
GTGTAGG59700.014.6949441
CTATACT102350.014.5921714
GTATAGG52800.014.4182421
GTACTAG40850.014.3763911
TCTATAC99200.014.2515953
GTGTAGA79450.014.1447321
ATGCCGG59450.014.025267145
TACACAG139400.013.8863795
TACACTG110850.013.800265
GTCCTAG56550.013.7185461
TAGACTG88350.013.7040985
CTTACAC83050.013.61838153
TACACCG16850.013.3383565
TACACGG19650.013.2825195
GTCTAGG62750.013.1718881
GTGTATA159350.013.0583041
TAGACAG166900.012.9449535
Produced by FastQC (version 0.11.5)