FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n01_MG_30907.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n01_MG_30907.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22963086
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG262800.017.2421845
TACACTG200450.016.854565
TACACCG32500.015.1692155
CTACACT151400.014.8447694
GTGTAGG113200.014.6671111
GTATAGG97900.014.2932211
CTATACT171700.014.1030724
CGTACAC29450.014.0321843
TACACAG254200.013.6899955
GTATTAG167050.013.6716091
CTTACAC150150.013.6645953
CCTATAC98850.013.4951223
CCTACAC111100.013.1164913
ATACTGT330600.012.9386126
TCTATAC179400.012.8511013
TCTACAC152650.012.8233843
GTATAGA165750.012.7727621
GTGTTAG175300.012.7386771
CTAGGAC101300.012.667753
GTGTAAG154150.012.6521491
Produced by FastQC (version 0.11.5)