FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n01_MG_30905.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n01_MG_30905.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15000994
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG35300.016.8417975
TATACTG168450.016.1832665
CTATACT111150.015.9810734
TACACTG115250.015.795
AGACGCG40600.014.643677145
GTGTAGG72450.014.4109851
GTATAAG105550.014.2881071
CCTATAC69250.013.924553
CTACACT91100.013.8477814
GTGTAGA100400.013.5044671
TCTATAC111050.013.3839593
ATACTGT213300.013.2223766
CTACCCT73150.013.0830494
CCTACAC73900.013.0483783
TCTACAC101350.013.019583
TAGACAG162700.012.9674555
GTAAGAC82300.012.9499113
CTTACAC90750.012.9424863
GTCCTAC48850.012.9129141
TATACCG28900.012.794455
Produced by FastQC (version 0.11.5)