FastQCFastQC Report
Sun 17 Mar 2019
H2YVLBGXB_n01_MG_30890.TR2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n01_MG_30890.TR2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18242917
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG60500.017.4960425
TACACTG120350.016.265225
GTGTAGG91900.015.0690251
TATACTG157950.015.00964455
GTCCTAG62000.014.50097751
GTGTAAG104650.014.2030591
CTATACT117250.014.0363864
TCTATAC123950.013.7455983
CTACACT96600.013.6595554
GTATAGA123950.013.6293991
TATACCG41400.012.9590665
CTTACAC98750.012.9216463
GTATAAG113500.012.9039541
TACACAG152750.012.8151845
GTATAGG73850.012.7632171
GTATTAG128500.012.7518331
CCTACAC91900.012.701433
TCTACAC119500.012.6193713
CCTATAC78650.012.5366783
CTAGTAC52350.012.4643063
Produced by FastQC (version 0.11.5)