FastQCFastQC Report
Sat 16 Mar 2019
H2YVLBGXB_n01_MG_30852.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n01_MG_30852.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14970274
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG159300.016.4754875
GTATAGG63850.016.0108831
TACACTG118300.014.7699075
TATACCG24550.013.8800755
CTACACT90650.013.8364214
TACACCG32200.013.7347075
GTGTAGA98650.013.5231491
GTGTAGG72550.013.2914171
CTATACT110400.013.0686124
CTTACAC95500.012.9058953
GTACTAG45900.012.7946791
GTATTAG109950.012.6608551
CTAGCCT88550.012.3632844
GTTACAC70000.012.2215583
GTCTTAC83500.012.0693921
GTGTTAG106550.011.9761241
GTATAGA108200.011.927511
TAGACTG93250.011.8178985
TCTATAC110800.011.7781023
CTAGGAC61600.011.7696043
Produced by FastQC (version 0.11.5)