FastQCFastQC Report
Sat 16 Mar 2019
H2YVLBGXB_n01_MG_30852.TR2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n01_MG_30852.TR2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14147503
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGCG47800.018.960157145
CTACACT80200.017.5374814
TACACTG106650.017.4027945
GTATTAG96600.015.6117161
TATACTG140600.015.2632235
GTGTAGA85650.014.4754871
CTATACT101550.013.850384
CCTACAC63600.013.6793023
CCTATAC56600.013.5777973
GTATAGG56300.013.5221381
TAGACAG157800.013.1860525
GTGTAAG81300.013.1096531
GTCCTAG58200.013.0806931
CTAGGAC55000.013.0500563
GTGTTAG98150.013.0013011
GTCTAGG59700.012.9949271
TCTATAC96500.012.8472053
CTAGCCT84650.012.8470734
ATAGGAC56600.012.8092423
CTTACAC84500.012.784083
Produced by FastQC (version 0.11.5)