FastQCFastQC Report
Sat 16 Mar 2019
H2YVLBGXB_n01_MG_30849.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n01_MG_30849.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13220560
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG139100.017.6693975
CTATACT97000.016.443724
TACACTG95350.015.8158245
TACACCG29400.015.2895085
ATGCCGG66300.014.654354145
GTATAGG49950.014.5155461
CCTATAC50600.014.1851463
GTATTAG111200.014.08373451
GTGTAAG77400.013.9577091
GTGTAGG56550.013.8471371
GTATAGA88100.013.6615841
CTACACT79600.013.3891634
CCTACAC59600.013.3812153
CTTACAC77150.013.3444783
GTACTAG40300.013.31360151
CTAGGAC51950.013.2582813
GTCTAGG55350.012.9684011
TATACCG20300.012.8574725
CTAGACT65350.012.7585524
TAGACAG135500.012.5741225
Produced by FastQC (version 0.11.5)