FastQCFastQC Report
Sat 16 Mar 2019
H2YVLBGXB_n01_MG_30849.TR2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n01_MG_30849.TR2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15321049
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG108050.015.7683655
TATACTG153850.015.126945
TATACCG37300.014.3835635
CTATACT117950.013.830194
TCTATAC115800.013.7111823
GTGTAGG77800.013.6060611
CTACACT88600.013.5836964
TATACAG144300.013.3144465
GTATTAG119250.013.1935141
GTATAGG72800.013.0466511
GTCCTAG50200.012.8542121
TAGTACT82550.012.734874
TACACCG45550.012.7334235
GTGTAAG89500.012.7184971
ACAGTGC96400.012.33398158
GTATAGA124550.012.2246031
CTAGACT72250.012.1420244
ATACTGT209350.012.1210066
GTGCTAG66450.012.1111991
TAATACT200800.012.0594174
Produced by FastQC (version 0.11.5)