FastQCFastQC Report
Sat 16 Mar 2019
H2YVLBGXB_n01_MG_30786.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n01_MG_30786.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11403700
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG120700.016.1580095
CTATACT78950.015.1521644
TACACTG90750.014.7797795
TCTATAC81900.014.1637113
CCTATAC48450.013.9165043
GTATAGG46850.013.61851
CTAGACT58950.013.5285654
TCTACAC72650.013.47221853
TACACCG21100.013.4006245
GTGTAGG51850.013.1442341
TAGACTG71850.012.8150355
CTAGGAC48850.012.7636493
CTTACAC71100.012.746223
GTATTAG76850.012.73638251
TCTAGAC62650.012.7295363
GTGTTAG78850.012.6891811
CTACACT69750.012.5772014
GTATAGA84550.012.5197431
TATACAG106200.012.4930785
TCCGTAC16350.012.4159873
Produced by FastQC (version 0.11.5)