FastQCFastQC Report
Sat 16 Mar 2019
H2YVLBGXB_n01_MG_30783.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n01_MG_30783.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20053536
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG210200.015.5558965
TACACTG156300.015.5394995
GTATAGG82300.014.0075971
CTACACT122050.013.7816224
CTATACT144150.013.4290944
TACACCG46200.012.8683575
TACACAG195050.012.4523145
TCTATAC147200.012.3134923
GTATTAG143150.012.0039261
GTATAGA141200.011.861611
GTGTAGG95700.011.81895351
GTGTTAG136050.011.7243971
CTAGACT99000.011.7174984
CCTATAC85600.011.6883813
ATACTGT275000.011.65311056
CCTACAC97650.011.5824763
CTTACAC115250.011.575133
TCTACAC125750.011.5311083
CTACCCT102450.011.4644484
GTGTAGA127200.011.4571161
Produced by FastQC (version 0.11.5)