FastQCFastQC Report
Sat 16 Mar 2019
H2YVLBGXB_n01_MG_30783.TR3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n01_MG_30783.TR3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14996024
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG38000.016.7894235
TATACTG140300.016.0191975
TACACTG111250.015.6404035
CTAGTAC44450.014.6792233
GTATAGA102300.014.599711
TACACAG137550.014.3892715
GTGTAGG69250.014.3434431
CTATACT102600.014.1325124
TCTACAC94500.014.11621953
CTACACT86850.014.1076154
GCTACAC68500.014.076463
CTTACAC82550.013.9640833
TAGGACT82100.013.599234
GTCCTAG53200.013.4919961
CTACCCT70650.013.340374
CTAGGAC53350.013.3175413
GTACTAG41950.013.1351271
TCTATAC105650.013.0381653
CTACACA108950.012.9760974
TAGACAG157350.012.9472135
Produced by FastQC (version 0.11.5)