FastQCFastQC Report
Sat 16 Mar 2019
H2YVLBGXB_n01_Lib_prep_NC_03142019.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVLBGXB_n01_Lib_prep_NC_03142019.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT11.0752688172043012No Hit
ATAATTAGTCCCCACCAGCTGCCAACCCAAAAGAGAAACAGCAGATAGCA11.0752688172043012No Hit
ACCCAGATCGTCCTCGACAACCGACTCCATCATAGTCAGCAGCTGAGCCG11.0752688172043012No Hit
CGTCTCTACTTTCCAAAACATCTAAAAGGCCAACAAAAATGTAGGATATT11.0752688172043012No Hit
GTTTGAGCCACTGCACCTGGCCCAAAAATTGCTTTAAAAAATATATATAG11.0752688172043012No Hit
CTTTTTACAGACTGCGCAAGGGTTCGTCCAGCACCTCGTCATGGGGCGCC11.0752688172043012No Hit
ACTCCAGATTAGGCAACATAGCGAGACACCATCATCTTTATAAATAAATG11.0752688172043012No Hit
TCGACGAACTCTACGAGGCCGTCATTGCGGGGCAGGCGCCTCTTCATGAC11.0752688172043012No Hit
GTCTTTAGCCATTCCATGAGAACCTCAAGATCAGTGTTCTTCCCTGCAAA11.0752688172043012No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAATGGCC11.0752688172043012No Hit
GGGCCATAATTTGGCTCAAACAAAAAAAAAATAAAACTAATAATAATAAA11.0752688172043012No Hit
GTACGGTGTGGTCCAAATGATTTGCTAACGTGGGAAAGGGGATATGCTTG11.0752688172043012No Hit
CTCTATCGTTGCCTCGTCCAGTTGTAATGACCGAATCATCTTGGAATATA11.0752688172043012No Hit
GGTATTTGCATCTGTCCGATCGGGCAATGGTGCGAAGCTATCATCTGCTG11.0752688172043012No Hit
TTATGATAGACTTTCAGCAACTAAAGTAAGCATTGACATTGAAAACACGG11.0752688172043012No Hit
GCTCTGGGGCTGTTGATATGGGCACAAGTGGAAGTGTGAAGGTAGAGCAT11.0752688172043012No Hit
AAACTTATCTGCTGGGACTGTCCTTAGCATCAGTTCTCTTTCTGTCTTCT11.0752688172043012No Hit
GTGGTGGACGACGTGCCGGCGCGGCACGCCGAGCTGATCGCCGCAGGTTA11.0752688172043012No Hit
AACAACCTCCATCGTTTCAATACACGAGTTTTCAAAAATACCAGGATGGG11.0752688172043012No Hit
AACCTGGGAGACGGAGGTTGAAGTGAGCCGAGATTGTGCCACTGCACTCC11.0752688172043012No Hit
GGTACAAGGGGATATATGCAATGGAAGTTAAATCTATGTTTATTTTTCAT11.0752688172043012No Hit
CCTTGATCACTGACAAGTTGCAGGACTCATCTACCAAAAATGACTTCACC11.0752688172043012No Hit
ATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGACA11.0752688172043012No Hit
CTGTTAGAGTATCTCCTTTATGGTACATTTATTATAAATACAGTACATGT11.0752688172043012No Hit
CTGATGTCGTGAAGGAACAGGTAGCTGACAAGATAGCGAAAGGTGCTAGG11.0752688172043012No Hit
GGGGGGGTGGGAAGCACGATCAGCGAGAAGTTACGCTCTGGATATGAACC11.0752688172043012No Hit
TCTTGTAACCTTCCTTTGTAGCAGCAGCACGAGCAGCACCCTTTTGCTCA11.0752688172043012No Hit
CTTCTAACCGACGTCGAAACGTACGGACTCTATATAAACCCGTCAGCCCC11.0752688172043012No Hit
CTTCCGATTTGTGCCGGGCCGGGTGAGGTTTCCCGTGTTGGGTCAAATTA11.0752688172043012No Hit
ACCTCACGCAGGGAGCGGCCCTGACGCTGGCGAACATAGCCAAGAACCTC11.0752688172043012No Hit
CCTGTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGCATGGGGGCACCC11.0752688172043012No Hit
GGTGGAGACACATCTATTGGTGCTATGGCAATAGTACAATCATTTATGAC11.0752688172043012No Hit
GCTAAAAACTGATTCTTTTCAAGAAAAATTAAAAAGGACGATTAAAAAAC11.0752688172043012No Hit
CCCAGCTACTCCGGAGGCTGAGGCAGGATAATGGCGAGAACTCGGGAGGC11.0752688172043012No Hit
AATAAACATTTCTCCAAGTAGATGTATGAATGGACAAGAAGAACAAGATC11.0752688172043012No Hit
GTCCAATTTAAAACCAATGTTCCTTTGTTAATTTTCCGTCTCAGTGATCT11.0752688172043012No Hit
CGTCTTGGCGGTCAGCTCCATCTGCGCTCGGGTGGCGGTCTCCGCGTGCT11.0752688172043012No Hit
CGCTTAGGCTTTCCGCTGGTGGGGACATCTGGGGGACAAGATCACCTTAT11.0752688172043012No Hit
GTATATATACTATATATGAATTTATAAAATCAGGATAGTAGCAGATTAGA11.0752688172043012No Hit
AAAAAATAGAAAACAATTAAATTCGATAATATAATATAACTATAATTTAT11.0752688172043012No Hit
ATCAACAAGTTCTGCGGGGATCCCACGCTGTTCGACGACGGCTTCAGGCT11.0752688172043012No Hit
CCTCGCGGGCGCGCTGGGCGGCTTCGGATTCGACCTTGCGGACGGCCTCT11.0752688172043012No Hit
ATAGAGAAGCAGTACTGTGAAGGAAAGGTGAAAAGAACCCCGGGAGGGGA11.0752688172043012No Hit
ATTTCACTCCCCTCCCGGGGTACTTTTCACCATTCCCTCACGGTACTATC11.0752688172043012No Hit
TGTGATGGTTTTTCAAAAGCTAACAATAAAACTACTTGTCTTATAAAGAA11.0752688172043012No Hit
CTTTAAAATCGTCTCTGTTATTGGCACATTGGTCGTATATCTGTTTTATA11.0752688172043012No Hit
TTGCATCCGGGGGGTTGCAAATGCTCTCCTTTATAGCTTCCCTCTCTCAG11.0752688172043012No Hit
GGGGCAAAGCTCCCTTCCTATAGTATGGCATATTGAGACGGTGTTAGAGA11.0752688172043012No Hit
ATAATACCCTGTATGGAGTCCTGTAAAACCACTCCCACCGGCACGGGGGT11.0752688172043012No Hit
GGGGGGGGGGGGGGGGCCTACAAGCCATAGAGTAAAGGCTCGCATGAATG11.0752688172043012No Hit
GGTCTGTTCTTTCACTTAAACTTTATTGTTATCACATACAAAGGTTGACA11.0752688172043012No Hit
CTATAAATGAAAATAAAATATAAGTAATAACTATATTTACTAAAAAAAAA11.0752688172043012No Hit
TCTCAAAGCTATATGCTTAATTAAAATAAAAGGGTGATATACATAATTGC11.0752688172043012No Hit
GTATTTACTATAATATCATAATTTAATAGTAGAAAAAGCAGCACCAACCT11.0752688172043012No Hit
AGCCTATTGCGACGAAGAAAACTTAGAGTGCACCGTTGTAGCTGATGTAA11.0752688172043012No Hit
CTGCTCTATCAATAGGAATGTTCAACTCTGTGAGGTGAATGCAATCATCA11.0752688172043012No Hit
GCTGTAGATTTTAGCTTTTTACATTTCTCTGTGTGCTGTTATGTTTCACA11.0752688172043012No Hit
CGGGTATCCAGGCAATGTCTTACTGCTGGACAAATACTGAGTAAATACAT11.0752688172043012No Hit
CCCTGATCCAGCTGGACATGTCCGAGTTTTCGGAGAAGCACACGGCGTCG11.0752688172043012No Hit
GGTCCTTGCTTTTATCACAGAACCTTCAAATATATAATAAAGTCATTCCT11.0752688172043012No Hit
CGCTTACGCTAACCCCATCAATTAACCTTCCAGCACCGGGCAGGCATCAC11.0752688172043012No Hit
GGTTAAATAACAAATTTTGTTATATGTGTTTTGCCATATTTTAAAAAAAT11.0752688172043012No Hit
GACAGACACAGTGAGACAGGCAGAGAGGGAGAGAGAGGGACAAAGACAGA11.0752688172043012No Hit
CCTCGGTACATCTCCTAAAAGTGCTTAAGTCTTAAGCTAAAGCAAGGTAA11.0752688172043012No Hit
GTCTGCGCCTGGTGCTCGGCAAGCGCGAGGGCTACGGAACCGTCAACATG11.0752688172043012No Hit
GTCCCACGCTGGGAAAGTGGTACTCTTTTAGAATAAAGAAGGGCTCATGG11.0752688172043012No Hit
CTTTTTAGTATATTCAGGATAAAATTTTGATGATTGGTCCCCTAATTTCT11.0752688172043012No Hit
GAGTAGGCCTCTGGGGAAGAGGATGTGGGGTGAGCGTGGTTTTGATACCC11.0752688172043012No Hit
CATGTACCCAAAAACTAAAAGAAAAAAAATAATACAAGAAAAAAAAAAAA11.0752688172043012No Hit
CAAGTGTGCAGGTCCTGGAGGGGGGCCTCCCCTGCCTCTGGCTTCCTTCT11.0752688172043012No Hit
CCCCTAAGGTTATACCTAATAACAAACTAATAGTAGAACATGGTAAAGCT11.0752688172043012No Hit
GTACCGAACTTGTGCTTTAACATTAGACATTTATTGTTTCACATTTTTCG11.0752688172043012No Hit
CAGCAACAGCATACTATATTTATACATTCAAACTAAATATAACTAAGTCT11.0752688172043012No Hit
GTGGTCGATGGCGGTATCGAGCCGCAGACCCGGCAGGTGCTGGAAAACGT11.0752688172043012No Hit
GTGGGAGGTTGTTAGGGGATAATATTGCTTCTTTTCCCTTCTATCTTCGC11.0752688172043012No Hit
GCCCTCTGTTGATTTGTGTTTACTCCAATTCTGGTTCTTAGGTCCCAGTA11.0752688172043012No Hit
GGGTCACGCACGTTATCTTAGTAGATTCTGCCATTGTAGACATTTTTAAT11.0752688172043012No Hit
TAAGGAGATTTAGTGATATTAAACAAGATTCATATAACCAAATGCCTGTT11.0752688172043012No Hit
CTAGAAAGAAAAGAGCAAAATAAAACCAATACAAGCAAAAGAAAGAAAAT11.0752688172043012No Hit
GGGGAAGACGTGTGCTCTTCCGATCTCCATGGGGAAGACGTGTGCTCTTC11.0752688172043012Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTACAGAACTGACACAGAGAGTTTGAGTAATTTGTACATGAACAAACATA11.0752688172043012No Hit
GTGTAGGATAGGTGGGAGACTGTGAAGCGGGCACGCCAGTGTTCGTGGAG11.0752688172043012No Hit
GGTCTGCGTCAAGGCCGCATGTTTTATATGAGCGCCAACGACCTTATCCT11.0752688172043012No Hit
GCAAAAGCAGTGGTTTAAAATGAATCCAAATCAGAAAATAACAACCATTG11.0752688172043012No Hit
AGCCAGGGAATTAATGGATAATTTGCAGGGAATAGCTTGTAGCGGAGTTG11.0752688172043012No Hit
GTGCTCTTCCGATCTGTCCTGGGAGACGTGTGCTCTTCAGTTCTGTCCTG11.0752688172043012No Hit
CACTTCCCCAGAGAGGGTGCAAAAGAAGCCACCCTCATGATCCAGTGCCA11.0752688172043012No Hit
GTCCAGGAGGTTGAGGCTACAGTGAGCTATGTTCATGCCCTGTCTCTTAT11.0752688172043012No Hit
GGGTCATTCTCTGTGGATCACAGTTTTGTATTTTTTCCTGAAAAGAAAGA11.0752688172043012No Hit
GTTCTGGGATGCCATAAAGGCAGTTTCATTCCATCAGAAAACTGAGACAC11.0752688172043012No Hit
CTTCCGATCTAAACTGGGAGACGTGTGCTCTTCCGATCTAATGTGGGAGA11.0752688172043012Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTGCATGCATCTACACAACTAGAAGGATTTTCAGCTGAATCAAGAAAAC11.0752688172043012No Hit
GATGAAACAATTTGACAATTTACCTATATCAAAATTATTAGAACCCAAGT11.0752688172043012No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCAGAA50.0145.0145
GAAGACG150.048.3333324
AAGACGT150.048.3333325
GGAAGAC150.048.3333323
CGTGTGC150.048.3333329
GGGAAGA150.048.3333322
GGGGAAG150.048.3333321
ACGTGTG150.048.3333328
AGACGTG200.036.256
GACGTGT200.036.257