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        Note that additional data was saved in multiqc_data when this report was generated.

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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.0.dev0

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2019-03-16, 00:03 based on data in: /beegfs/mk5636/logs/html/H2YVKBGXB/merged

        General Statistics

        Showing 15/15 rows and 3/5 columns.
        Sample Name% Dups% GCM Seqs
        H2YVKBGXB_n01_mb185
        10.3%
        42%
        38.6
        H2YVKBGXB_n01_mb186
        31.6%
        41%
        27.7
        H2YVKBGXB_n01_mb187
        18.3%
        41%
        29.3
        H2YVKBGXB_n01_mb188
        30.4%
        40%
        30.5
        H2YVKBGXB_n01_mb189
        23.8%
        41%
        36.3
        H2YVKBGXB_n01_mb190
        14.0%
        44%
        36.6
        H2YVKBGXB_n01_mb191
        9.4%
        48%
        37.6
        H2YVKBGXB_n01_mb192
        14.6%
        46%
        26.4
        H2YVKBGXB_n01_mb193
        6.6%
        48%
        31.4
        H2YVKBGXB_n01_mb194
        53.5%
        48%
        30.9
        H2YVKBGXB_n01_mb195
        55.4%
        49%
        33.6
        H2YVKBGXB_n01_mb197
        51.5%
        50%
        30.0
        H2YVKBGXB_n01_mb198
        65.5%
        51%
        28.8
        H2YVKBGXB_n01_mb199
        57.3%
        51%
        29.0
        H2YVKBGXB_n01_undetermined
        65.2%
        44%
        30.5

        Demultiplexing Report

        Demultiplexing Report
        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool.

        Showing 15/15 rows and 2/2 columns.
        Sample NameTotal Read CountPortion (%)
        undetermined_library
        30,484,208
        6.4
        mb185
        38,554,301
        8.1
        mb186
        27,690,025
        5.8
        mb187
        29,276,522
        6.1
        mb188
        30,484,381
        6.4
        mb189
        36,344,911
        7.6
        mb190
        36,622,163
        7.7
        mb191
        37,596,723
        7.9
        mb192
        26,419,073
        5.5
        mb193
        31,370,253
        6.6
        mb194
        30,908,606
        6.5
        mb195
        33,554,754
        7.0
        mb197
        29,992,667
        6.3
        mb198
        28,838,937
        6.0
        mb199
        28,969,343
        6.1

        Barcodes of Undetermined Reads

        Barcodes of Undetermined Reads
        We have determined the barcodes of your undetermined reads (reads containing a barcode that you did not encode in your metadata). Here are the top 20 barcodes belonging to the undetermined reads. The full list is available here.

        Showing 20/20 rows and 2/2 columns.
        Sample NameCountFrequency (%)
        GGGGGG
        23177484.0
        76.0
        TTAGCA
        168494.0
        0.6
        GATGTA
        162874.0
        0.5
        ATCAGA
        145916.0
        0.5
        ACTGAA
        128780.0
        0.4
        ACAGGA
        109467.0
        0.4
        CGATTA
        99960.0
        0.3
        AAGTGA
        91577.0
        0.3
        GCCATA
        90981.0
        0.3
        ATCCGA
        89107.0
        0.3
        GGGGGT
        86121.0
        0.3
        TTGTAA
        85930.0
        0.3
        ATTGAA
        83523.0
        0.3
        TACCAA
        80543.0
        0.3
        TCACGA
        78290.0
        0.3
        GCAATA
        77763.0
        0.3
        AGATCA
        77128.0
        0.2
        ATACGA
        74396.0
        0.2
        CCCGTC
        73689.0
        0.2
        TGCCAA
        73135.0
        0.2

        Run Statistics

        Run Statistics

        Showing 1/1 rows and 4/4 columns.
        Sample NameTotal # of Single-End ReadsTotal # PF Reads% Undetermined % PhiX Aligned
        4.0
        520,560,384
        477,106,867
        6.4
        3.9

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Quality Histograms

        The mean quality value across each base position in the read. See the FastQC help.

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        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality. See the FastQC help.

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        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called. See the FastQC help.

        Click a heatmap row to see a line plot for that dataset.

        rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content. See the FastQC help.

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        Per Base N Content

        The percentage of base calls at each position for which an N was called. See the FastQC help.

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        Sequence Length Distribution

        All samples have sequences of a single length (76bp).

        Sequence Duplication Levels

        The relative level of duplication found for every sequence. See the FastQC help.

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        Overrepresented sequences

        The total amount of overrepresented sequences found in each library. See the FastQC help for further information.

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        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. See the FastQC help. Only samples with ≥ 0.1% adapter contamination are shown.

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        MultiQC v1.0.dev0 - Written by Phil Ewels, available on GitHub.

        This report uses HighCharts, jQuery, jQuery UI, Bootstrap, FileSaver.js and clipboard.js.