FastQCFastQC Report
Sat 16 Mar 2019
H2YVKBGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVKBGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30484208
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG605550.19864383552296977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC163800.052.6217113
CGTCTGA161750.051.88384616
ACGTCTG163450.051.3432115
CACGTCT166250.051.3417114
CACACGT175150.049.6712912
GCACACG179650.048.99184811
GATCGGA210900.047.370291
ATCGGAA214750.047.038542
CGGAAGA209950.046.7392354
TCGGAAG212600.046.3889243
GAACTCC203850.039.92251621
AGAGCAC235300.039.3688668
AGCACAC240400.038.09563410
CTGAACT222300.037.81961419
TGAACTC237250.034.93520720
AACTCCA246950.032.70010822
CTCCAGT225250.029.85198424
CCAGTCA188500.029.00435426
TCTGAAC313500.027.32959718
CAGTCAC179900.025.91506827
Produced by FastQC (version 0.11.5)