FastQCFastQC Report
Sat 16 Mar 2019
H2YVKBGXB_n01_mb199.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVKBGXB_n01_mb199.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28969343
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTA8850.014.2365773
CCCCCGT38850.012.7948921
CTCCCGT72150.012.2750571
CTCCGAT64050.012.0784851
CTCGCGT10100.011.7841121
CCCGTAT40950.011.7113241
CTCCGTT82400.011.4703291
CGTTTCG26750.011.25348431
CCCCGGT46250.010.9747741
CCCGTTT78650.010.9490311
TCCGCTA12800.010.9370292
CGCCTAT10600.010.8980151
GTCCGGT31850.010.7709991
CGGGCTA17300.010.7243041
GTACGAC31400.010.47879425
CTCCGGT101300.010.4706231
TACGACT38250.010.3392646
TACGCTA23800.010.29465237
CCCGATT29250.010.2922881
CGCTACT14850.010.1363441
Produced by FastQC (version 0.11.5)