FastQCFastQC Report
Sat 16 Mar 2019
H2YVKBGXB_n01_mb198.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVKBGXB_n01_mb198.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28838937
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCCGG109950.020.43532670
CGACTAA10450.019.0898864
CGTATTA7150.018.110983
CCGACTA14350.017.5600973
TACGACT101450.016.3174516
GACCCCG135950.016.26970169
GGCCGAC152650.015.63618665
GACGTAT18250.015.53435717
GTTACGA110150.015.2511114
TTACGCG2858.566349E-814.73592456
CGGGCTA12800.014.2207981
CGTTTCG51300.014.1922931
CGCTAGT10200.014.0706541
TTACGAC122550.013.7936315
CCCCCGT28950.013.6634521
CGGAAAC58150.013.4241171
GGGCCGA175150.013.20794764
CTCGCGT10350.013.1903061
CTCCCGT63850.013.157731
TCGACCG187450.013.01528931
Produced by FastQC (version 0.11.5)