FastQCFastQC Report
Sat 16 Mar 2019
H2YVKBGXB_n01_mb197.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVKBGXB_n01_mb197.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29992667
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTAT12750.011.8053911
CGTATTA10650.011.1732373
CCCGAAT60200.011.0478871
CTCCGCT106150.011.014091
CGCTAGT11000.010.8195291
CTCCCGT79300.010.6381461
CTCGCGT12250.010.5727451
CTCCGTT82400.010.5352921
CTCGTAT57400.010.4281281
GTCCGGT39950.010.339211
CTCCGGT102600.010.3375441
CCCGTTT78950.010.2419341
CGTTTCG25850.010.155580531
CCCCCGT42500.010.1306731
CTCCGAT77350.09.9107351
CCCGATT36450.09.8914931
GTCCGTT44750.09.7777541
CCCGTAT36500.09.6861381
CCCGCAT54000.09.6586091
CCCCGAT66800.09.4847141
Produced by FastQC (version 0.11.5)