FastQCFastQC Report
Sat 16 Mar 2019
H2YVKBGXB_n01_mb195.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVKBGXB_n01_mb195.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33554754
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACT74500.016.535956
ACCCCGG100650.016.06458970
CGTATTA13750.016.0355953
TGTTACG79250.015.2358653
GTTACGA85850.014.5536714
GACGTAT25600.014.49250917
TTACGAC87650.014.3346535
GACCCCG112100.013.83055269
GGCCGAC117050.013.75398365
CGCTAGT11900.013.2374611
CGTATAG31100.012.94358419
TCGACCG126750.012.81357531
CCCGTAT40650.012.6588981
ACGTATA30150.012.65447118
CGGGCTA17000.012.5608811
CTCCCGT79300.012.3601581
GGGCCGA133500.011.98055764
CGACCGT138700.011.86104632
CTCCGAT91500.011.8598651
CGTTTCG35250.011.61788431
Produced by FastQC (version 0.11.5)