FastQCFastQC Report
Sat 16 Mar 2019
H2YVKBGXB_n01_mb194.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVKBGXB_n01_mb194.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30908606
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTA16500.018.8780423
CCCGTAT41900.015.4557761
GACGTAT25950.014.97125717
CGTATAG29300.013.38002319
TTAACCG22350.013.3102437
ACGTATA29600.013.125627518
TAACCGT24800.012.701078
CGCCTAT14150.012.6167311
CTCCGAT90550.012.602661
CTCCCGT72850.012.4452421
CTCGCGT9650.012.3334541
CCCCCGT35300.011.7014691
CTCCGGT94300.011.6931631
ACCGTTA24850.011.68962210
CCCCGGT46600.011.6433751
GACTACG34100.011.49652335
CTCGTAT58150.011.1366631
CTCCGTT89850.010.8307821
CCGTTAA26200.010.82037611
GTCCGGT32450.010.787441
Produced by FastQC (version 0.11.5)