FastQCFastQC Report
Sat 16 Mar 2019
H2YVKBGXB_n01_mb193.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVKBGXB_n01_mb193.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31370253
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT10300.030.24088342
TCGCGTA11150.028.56338141
ATCGCGT19400.028.50396740
TGAATCG76200.028.43302337
AATCGCG34400.028.1824639
GAATCGC44500.022.65135838
AATCGGG57050.019.57008239
CGTCTGA207800.018.94865616
GAATCGG62450.018.49452838
ACGTCTG227050.017.41887515
TTGAATC182400.017.2322936
ACACGTC241850.016.43965113
CACGTCT248900.015.97413214
TCGTATG26900.015.872642542
CTCGTAT26650.015.36496941
AATCTCG35400.015.32449139
GTCACAC258600.015.2956229
CTTGAAT228800.014.70171235
TCGCGTT16950.014.24696741
ATCGGGT34700.014.12045140
Produced by FastQC (version 0.11.5)