FastQCFastQC Report
Sat 16 Mar 2019
H2YVKBGXB_n01_mb192.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVKBGXB_n01_mb192.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26419073
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC1533790.5805616268216527TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCGCGTATGC284590.10772141778025292TruSeq Adapter, Index 3 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG203850.063.1287943
CTCGTAT202950.062.8569142
TCTCGTA201000.062.5961241
CGTATGC234700.062.55520244
TATGCCG242900.061.2070646
CGCGTAT45600.061.0931942
ATGCCGT229900.060.6338447
CGTCTGA386950.060.28538516
ACACGTC390300.059.93708413
ATCTCGT226900.059.56984740
ACGTCTG394450.059.20034815
TCGCGTA43700.058.94418341
GATCGGA410550.058.446031
ATCGGAA412400.058.430182
TGCCGTC229500.058.17757448
CACGTCT403500.057.96808214
CATCTCG247450.057.2827239
CCGTCTT214100.056.59209450
TCGGAAG429500.055.997823
CACACGT418750.055.92344712
Produced by FastQC (version 0.11.5)