FastQCFastQC Report
Sat 16 Mar 2019
H2YVKBGXB_n01_mb191.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVKBGXB_n01_mb191.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37596723
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC409580.1089403456785316TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT89550.034.8617542
TCGTATG97300.034.5308943
CGTATGC100500.033.70999544
TCTCGTA90000.033.5987841
TATGCCG103200.032.7940546
ATGCCGT103950.032.3553847
ACGATCT100500.029.81192437
ATCTCGT103800.028.76134140
CACGATC105450.028.47936
TGCCGTC120750.027.91173248
CGATCTC107850.027.9091238
TCACGAT115750.027.88059435
GATCTCG109500.027.45628239
ATCACGA122800.027.4202334
CCGTCTT123800.026.79999750
GCCGTCT129850.025.65917249
CATCACG141550.024.38159233
CGTCTTC138150.023.94021251
GTATGCC149250.022.95707745
GCTTGAA201500.017.24737559
Produced by FastQC (version 0.11.5)