FastQCFastQC Report
Sat 16 Mar 2019
H2YVKBGXB_n01_mb190.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVKBGXB_n01_mb190.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36622163
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC6807281.858786986448616TruSeq Adapter, Index 12 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATGC1523590.4160294955816783TruSeq Adapter, Index 12 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG966450.066.0129943
CTCGTAT797600.066.0034142
TCTCGTA792900.065.97127541
CGTATGC996650.065.8561944
ATGCCGT961550.065.5299147
TATGCCG1000900.065.366446
AATCTCG828100.065.2188739
TGCCGTC949100.064.96973448
CCGTCTT900750.064.6571350
ATCTCGT827700.064.6283540
GCCGTCT884350.064.6017449
CGTCTGA1225950.064.3017116
ACACGTC1235650.064.0042813
ACGTCTG1237100.063.7519815
CGTCTTC872450.063.6656251
CACGTCT1254500.062.9957314
GTATGCC1055250.062.91179345
GTCGTAT204100.062.3486242
ATCGGAA1305100.061.7968832
TGTCGTA205750.061.64483641
Produced by FastQC (version 0.11.5)