FastQCFastQC Report
Sat 16 Mar 2019
H2YVKBGXB_n01_mb189.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVKBGXB_n01_mb189.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36344911
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC2984570.8211796143894808TruSeq Adapter, Index 7 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTATGC1293800.3559783101408613TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTTTGC472430.12998518554633412TruSeq Adapter, Index 7 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGG417580.1148936642051483TruSeq Adapter, Index 7 (97% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA202200.065.63508641
CGCGTAT209100.065.47729542
ATCGCGT276050.065.11648640
CATCGCG342600.064.91139
CGTCTGA1293800.064.46216616
ACACGTC1300900.064.42657513
ACGTCTG1301400.064.13090515
CGTATGC505850.063.9424944
CACGTCT1312800.063.6967414
TATGCCG536250.063.61980446
TCTCGTA422150.063.3396241
CTCGTAT424100.063.22125242
TCGTATG402900.063.099243
GCACACG1333000.063.0377311
CACACGT1338900.062.66070612
ATGCCGT449650.062.64860547
CATCTCG571150.062.51634639
ATCTCGT517250.062.41939540
ATCATCG475600.062.35600737
CGGAAGA1376100.062.203964
Produced by FastQC (version 0.11.5)