FastQCFastQC Report
Sat 16 Mar 2019
H2YVKBGXB_n01_mb188.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVKBGXB_n01_mb188.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30484381
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC3539891.1612143280849296TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCAAT437500.064.25249533
CGTATGC412250.064.0026644
TCTCGTA409200.064.0007641
TCGTATG408850.063.9870743
CTCGTAT408250.063.94394342
GTCACGC445050.063.58938229
TATCTCG418700.063.41960539
TATGCCG417000.063.29030646
ATGCCGT412250.063.077247
ACGCCAA448250.062.9937332
ATCTCGT423850.062.57419240
TCACGCC451700.062.559330
TGCCGTC411950.062.49445748
CGTCTGA462550.062.1306516
ACACGTC466650.061.97336213
AGTCACG458500.061.8760828
GCCGTCT400000.061.5966349
CACGCCA458600.061.5951431
CCGTCTT403400.061.5113650
ACGTCTG470300.061.20214515
Produced by FastQC (version 0.11.5)