FastQCFastQC Report
Sat 16 Mar 2019
H2YVKBGXB_n01_mb187.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVKBGXB_n01_mb187.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29276522
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC1725920.5895235779714544TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1250940.42728436116831087TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGTATGC541130.1848341138336036TruSeq Adapter, Index 5 (97% over 41bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC447900.063.76038444
ACACGTC724000.063.50153413
TATGCCG452350.063.35729646
CGTCTGA721850.063.3135616
TCGTATG371900.063.24872643
ACGTCTG724800.063.084315
TCGCGTA40950.062.90304641
GATCGGA753750.062.728141
ATGCCGT410950.062.72260747
ATCGGAA757850.062.5109832
TGCCGTC393650.062.41176648
CACGTCT735850.062.3223814
GTCGTAT233050.062.18729442
TCGGAAG760550.062.0820923
GCACACG746900.061.77939211
CGGAAGA763000.061.7262654
GCCGTCT340350.061.6868749
TGTCGTA232600.061.49540341
CCGTCTT359100.061.4093450
TCTCGTA167950.061.14034741
Produced by FastQC (version 0.11.5)