FastQCFastQC Report
Sat 16 Mar 2019
H2YVKBGXB_n01_mb186.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVKBGXB_n01_mb186.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27690025
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC5038481.8196011018408251TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC640750.063.91712644
GATCGGA700850.063.8968351
CTCGTAT609900.063.8679642
TCGTATG638450.063.8405243
ACACGTC683500.063.77691313
TCTCGTA596450.063.69478241
ATCGGAA704550.063.648232
ATGCCGT636400.063.6056447
CGTCTGA682800.063.56791716
AATCTCG590450.063.46057539
TATGCCG643450.063.34387246
ACGTCTG686600.063.2146115
TGCCGTC636850.063.175948
TCGGAAG708550.063.012653
GCCGTCT621950.063.01251649
ATCTCGT596250.062.8715940
CCGTCTT626100.062.7851350
CGGAAGA711000.062.627934
CGTCTTC621250.062.62743451
CACGTCT695400.062.5705714
Produced by FastQC (version 0.11.5)