FastQCFastQC Report
Sat 16 Mar 2019
H2YVKBGXB_n01_mb185.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YVKBGXB_n01_mb185.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38554301
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC1219260.31624487239439253TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA55050.063.95700541
TATCGCG77650.062.87695339
CGCGTAT57600.062.03642742
CGATGTA344050.061.8777234
ATCGCGT68250.061.69010540
CACCGAT352350.060.90753631
ACCGATG352100.060.8416632
CCGATGT351900.060.5874633
CGTATGC200450.059.8693344
TCTCGTA175250.059.67192541
GTATCGC85250.059.53005238
CTCGTAT176600.059.512542
TCGTATG166850.059.403343
TATCTCG208150.059.22044439
ATGCCGT186750.059.16361247
GTCACCG368500.058.4377329
TCACCGA373700.057.63229830
TATGCCG217900.057.5961946
TGCCGTC184550.057.15706648
ATCTCGT205800.056.06942740