FastQCFastQC Report
Wed 20 Mar 2019
H2YNTBGXB_n01_9_input_BZIP1_BIO.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2YNTBGXB_n01_9_input_BZIP1_BIO.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8864996
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC300919833.944719207995135TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG4178614.71360618775237TruSeq Adapter, Index 9 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1271701.4345184137702938No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGC445950.5030459122598588TruSeq Adapter, Index 9 (98% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC326310.36808815254964583TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGCTCTCGTATGC273930.3090018314729076TruSeq Adapter, Index 9 (98% over 50bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG256490.2893289517558722TruSeq Adapter, Index 9 (97% over 49bp)
ACACTCTTTCCCTACACGACGCTCTTCCGAGATCGGAAGAGCACACGTCT211520.23860134849468628Illumina Single End PCR Primer 1 (96% over 30bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG211370.23843214368060628Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAGCTCGTATGC206030.23240845229935805TruSeq Adapter, Index 9 (98% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGT179240.20218847250466893TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGCTCTCGTATG152100.17157368147712643TruSeq Adapter, Index 9 (97% over 49bp)
GATCCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG134660.15190080176009105TruSeq Adapter, Index 9 (97% over 47bp)
GATTCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG129990.1466328918817335TruSeq Adapter, Index 9 (100% over 47bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATAGATCGGAAGAGCACACGT125520.14159058842214933Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGAAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC114980.1297011301527942TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCAGATCTCGTATGC114700.12938528116651152TruSeq Adapter, Index 9 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAGCTCGTATG113530.12806548361668746TruSeq Adapter, Index 9 (97% over 49bp)
GATCGGAAAAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC104380.11774398995780709TruSeq Adapter, Index 9 (98% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATG100240.11307393708919891TruSeq Adapter, Index 9 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAATCACGATCAGATCTCGTATGC96690.1090694231559721TruSeq Adapter, Index 9 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG3580950.068.27257570
AAAAAGG3987000.061.41989569
AAAAAAG4034250.061.2152468
TCTCGTA3977850.058.4002841
ATCTCGT3941300.058.3237940
CTCGTAT4025750.058.19640742
TGCTTGA4086750.058.03888758
GCTTGAA4089400.058.03241759
CTTGAAA4100850.058.0087960
TATGCCG4107000.057.9576746
TCGTATG4120850.057.94666343
AGATCTC3933800.057.9446638
CGTATGC4118000.057.93694744
GTATGCC4110800.057.922345
TTCTGCT4097000.057.92220355
CCGTCTT4094850.057.92068550
GCCGTCT4097500.057.90925249
TCTGCTT4098550.057.8405256
ATGCCGT4109750.057.8357647
TGAAAAA4134600.057.82032862
Produced by FastQC (version 0.11.5)